DTT (1,4-Dithiothreitol, AS-D0505): Essential Reducing Agent for Protein Analysis

Astor Scientific offers DTT (1,4-Dithiothreitol, AS-D0505), a high-purity reducing agent widely used in biochemical and molecular biology applications. This compound is crucial for maintaining the reduced state of thiol groups in proteins, facilitating accurate analysis and experimentation.

Product Highlights

  • Purity: >99%

  • UV Absorbance: <0.1 at 280 nm (1% solution)

  • Storage: Store at -20°C

  • Available Sizes: 1g, 5g, 10g, 50g

  • Price: Starting at $36.00

For more details and to purchase, visit the product page: .

Applications

  • Protein Analysis: Reduces disulfide bonds in proteins, aiding in structural studies.

  • SDS-PAGE: Prepares protein samples by breaking disulfide linkages.

  • Enzyme Activity: Maintains the active form of enzymes sensitive to oxidation.

  • Cross-Linking Reactions: Breaks disulfide bridges in cross-linkers like N,N′-bis(acryloyl)cystamine.

Advantages Over Alternatives

  • Less Toxic: DTT is less toxic and less pungent compared to 2-mercaptoethanol.

  • Lower Concentration Required: Typically, a seven-fold lower concentration of DTT (100 mM) is needed than 2-mercaptoethanol (5% v/v, 700 mM).

Why Choose Astor Scientific?

Astor Scientific provides high-quality reagents with exceptional customer service. Their platform offers a comprehensive selection of life science products, ensuring that researchers have access to the tools they need for their studies.

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